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ZO-1 expression
RPE-ZO1
ZO-1 immunolabeling
Immuno-fluorescence
Tight-junction, epithelial barrier
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Induction of sebogenesis by arachidonic acid
SEB-AAI
Cell ratio intensity of staining
Bodipy fluorescence
PPAR stimulation, sebogenesis (arachidonic acid induction)
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Androgen receptor activation
SEB-ARS
Cell ratio intensity of staining
BODIPY fluorescence
Androgen receptor stimulation, sebogenesis
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Cellular oxidative stress (sebocytes)
SEB-CRX
Cell ratio intensity of staining.
Fluorescent marker
Oxidative stress response at cell level
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Inflammation (expression)
SEB-ICE
IL-8, IL-6 and TNF-a mRNA expression
RT-qPCR
PPAR stimulation, inflammation (arachidonic acid induction)
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Inflammation (secretion)
SEB-INF
IL-8 concentration in cell supernatant
ELISA
PPAR stimulation, inflammation (arachidonic acid induction)
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Induction of sebogenesis by linoleic acid
SEB-LAI
Cell ratio intensity of staining
BODIPY fluorescence
PPAR stimulation, sebogenesis (linoleic acid induction)
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Cell proliferation/Survival (sebocytes)
SEB-LCC
Nb live/dead cells
Calcin AM - Annexin V*
Cell proliferation - Survival
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mRNA expression
SEB-RNA
Relative expression level. All genes
RT-qPCR
Sebogenesis, acne
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