Acne, Oily skin model and related in vitro assays

Acne model is based on human iPSC-derived sebocytes.
Identify active ingredients among hundreds on our innovative high-throughput assays.
Validate or study mechanism of action with dedicated bioassays.

Our high-throughput screening services for seborrhea dysfunction :
Starting price at 4 500€ / 96wp

This is a photo of three womans with different skin color . Their different ethnic skins origins symbolize our various skin cells models for dermo-cosmetic industry.

Ethnic specific
claim validation

Caucasian,
Asian and
African
Overall Protocol for Acne / Hyperseborrhea claims
Using High-Throughput Imaging (HTI)
Pattern of the high-throughput Imaging for sebocyte screening
first compound addition in the 96 well plate, then automated imaging to identificate activity and last valide the mechanism of action
Active ingredient discovery by high-throughput automated imaging
There is two line with three photos each. First line is Hoechst marking, the second is bodipy marking for fatty acid.
First column is the vehicle (normal cells). Second shows activation of sebocytes by Arachidonic Acid. last one is the negative control.
High-content readout:
Ingredient effect on total lipid production
the induction by arachidonic acid shows a real increase of sebum production compared to oher conditions.

Acne facts

Bioassays catalog for Sebocyte

Area
Name
Method
Endpoint
Ref.
Sebogenesis
Androgen receptor stimulation
BODIPY
Normalized fluorescence
SEB-ARS
Sebogenesis
PPAR stimulation by Linoleic Acid
BODIPY
Normalized fluorescence
SEB-LAI
Sebogenesis
PPAR stimulation by Arachidonic Acid
BODIPY
Normalized fluorescence
SEB-AAI
Sebogenesis
mRNA expression of characterized markers
RT-qPCR
Relative expression level
SEB-RNA
Sebogenesis
Perilipin (gene expression)
RT-qPCR
Relative expression level
SEB-RNA
Sebogenesis
Arachidonic acid receptor (PPARG)
RT-qPCR
Relative expression level
SEB-RNA
Sebogenesis
Triglyceride synthesis (DGAT2)
RT-qPCR
Relative expression level
SEB-RNA
Sebogenesis
Lipoprotein lipase (LPL)
RT-qPCR
Relative expression level
SEB-RNA
Sebogenesis
Sapienic acid synthesis (FADS2)
RT-qPCR
Relative expression level
SEB-RNA
Sebogenesis
Squalene synthesis (FDFT1)
RT-qPCR
Relative expression level
SEB-RNA
Sebogenesis
Wax ester synthesis (AWAT1)
RT-qPCR
Relative expression level
SEB-RNA
Sebogenesis
Sebocyte maturation (MUC1)
RT-qPCR
Relative expression level
SEB-RNA
Sebogenesis
Lipid droplet maturation (PLIN2)
RT-qPCR
Relative expression level
SEB-RNA
Oxidative stress
Cellular oxidative stress
CellROX staining
Normalized fluorescence
SEB-CRX
Inflammation
TNFα monitoring
RT-qPCR
Relative expression level
SEB-TNF
Inflammation
IL-8 monitoring
RT-qPCR
Relative expression level
SEB-IL8
Inflammation
IL-6 monitoring
RT-qPCR
Relative expression level
SEB-IL6
Cell proliferation
Cell proliferation / Survival
Calcein AM - EthD1
Living cells / Dead cells
SEB-LCC

Do you have specific R&D needs ?

We are able to do custom-made bioassays for you.

Move your R&D forward
With our innovative, efficient and cost-effective bioassays and cell models
Get in touch