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In this study, we describe a simple and straightforward assays using induced pluripotent stem cell-derived melanocytes ad high-throughput flow citometry, to identify the effect induced by pigment regulating agents on melanin content. The assay is based on the correlation between forward light-scatter characteristics and melanin content, with pgmented cells displaying high light absorption/low forward light scatter. While the opposite is true for lowly pigmented melanocytes, as a result of genetic background or chemical treatments. Orthogonal validation is then performed by regular melanin quantifications. Such approach was validated using a set of 80 small molecules and yielded a confirmed hit. The assay described in this stuy may prove a useful tool to identify modulators of melanogenesis in human melanocytes.