Cell stress and survival

Standard phenotypic assays
with Caucasian, African or Asian
iPSC-derived skin cells
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Cell stress facts

Cell stress-related bioassays

Name
Ref.
Endpoint
Method
Biological question
Apoptosis
RPE-TUN
Cell ratio intensity of staining
TUNEL Fluorescent staining
Apoptosis
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Mitochondrial Oxidative stress
RPE-MSX
Cell ratio intensity of staining
Fluorescent staining
Oxidative stress response at mitochondria level
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Cellular oxidative stress
RPE-CRX
Cell ratio intensity of staining
Fluorescent staining
Oxidative stress response at cell level
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Cell proliferation/Survival
RPE-LCC
Nb live/dead cells
Calcin AM - Annexin V*
Cell proliferation - Survival
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Cellular oxidative stress (melanocytes)
MEL-CRX
Cell ratio intensity of staining
Fluorescent marker
Oxidative stress response at cell level
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Cellular oxidative stress (sebocytes)
SEB-CRX
Cell ratio intensity of staining.
Fluorescent marker
Oxidative stress response at cell level
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Cell proliferation/Survival (melanocytes)
MEL-LCC
Nb live/dead cells
Calcin AM - Annexin V*
Cell proliferation - Survival
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Cell proliferation/Survival (sebocytes)
SEB-LCC
Nb live/dead cells
Calcin AM - Annexin V*
Cell proliferation - Survival
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Inflammation (secretion)
SEB-INF
IL-8 concentration in cell supernatant
ELISA
PPAR stimulation, inflammation (arachidonic acid induction)
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