Dry AMD model and in vitro assays

Our Dry AMD model combines blue light and A2E treatment of iPSC-derived RPE cells.
Screen small molecule librairies with high-throughput assays.
Test and validate your active coumpound with dedicated in vitro bioassays.

Our HTS services for Age-related Macular Degeneration :
Starting price 11 500 € / 96 wp

This is a photo, from a microscope, of staining RPE cells

Blue light exposure

Use our iPSC-derived in vitro model assay
to improve the relevance of
your ophthalmology dry AMD R&D 
Steps of ADM model making. First grow cells during two weeks. Then treat them with A2E during two weeks finally expose them during for day to blue light.This is a photo of automate dedicated to HTS a biology laboratory.
Screenable endpoint
Live/Dead in 96 wells plate
Experience to reveal the impact of blue light on RPE cells's death.
Graph showing the impact of A2E and blue light on the % of living cells.Legend of the graph bellow.

Bioassays catalog for RPE cells

Area
Name
Method
Endpoint
Ref.
Retinal disorders
VEGF (Growth Factor) secretion
OD reading
VEGF concentration
RPE-VEGF
Retinal disorders
PEDF (Growth Factor) secretion
OD reading
PEDF concentration
RPE-PEDF
Retinal disorders
Phagocytosis assay
Bioparticles labeling
Normalized fluorescence
RPE-PHAG
Retinal disorders
Outer retinal barrier resistance
Volt/Ohm meter
Resistance in Ohm/cm2
RPE-ORB
Retinal disorders
mRNA expression of characterized markers
RT-qPCR
Relative expression level
RPE-RNA
Pigmentation
Melanin content
OD reading
Melanin concentration
RPE-MEL
Pigmentation
PMEL17 expression
Immunolabeling
Normalized fluorescence
RPE-PMEL17
Pigmentation
TYRP1 expression
Immunolabeling
Normalized fluorescence
RPE-TYRP1
Oxidative stress
Cellular oxidative stress
CellROX Staining
Normalized fluorescence
RPE-CRX
Oxidative stress
Mitochondrial Oxidative stress
Immunolabeling
Normalized fluorescence
RPE-MSX
Identification
MITF expression
Immunolabeling
Cell population purity
RPE-MITF
Identification
ZO-1 expression
Immunolabeling
Cell population purity
RPE-ZO1
Cell proliferation
Cell proliferation / Survival
Calcein AM - EthD1
Living cells / Dead cells
RPE-LCC
Apoptosis
RPE Apoptosis
TUNEL staining
Normalized fluorescence
RPE-TUN

Do you have specific R&D needs ?

We are able to do custom-made bioassays for you.

Move your R&D forward
With our innovative, efficient and cost-effective bioassays and cell models
Get in touch