Retinal-related assays

PCi-RPE (retinal pigment epithelium)
cells display the full phenotypic, molecular and functional characteristics of in situ RPE with the advantage of unrestricted availability.

RPE-based bioassays

Name
Ref.
Endpoint
Method
Biological question
mRNA expression
RPE-RNA
Relative expression level. All genes
RT-qPCR
Retinal disorders
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TYRP1 expression
RPE-TYRP1
TYRP1 immunolabeling
Immuno-fluorescence
Pigmentation
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PMEL17 expression
RPE-PMEL17
PMEL17 immunolabeling
Immuno-fluorescence
Pigmentation
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ZO-1 expression
RPE-ZO1
ZO-1 immunolabeling
Immuno-fluorescence
Tight-junction, epithelial barrier
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MITF expression
RPE-MITF
MITF immunolabeling
Immuno-fluorescence
Key transcription factor
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Outer retinal barrier
RPE-ORB
Resistance in Ohm/cm2
Epithelial Volt/Ohm meter
Epithelial barrier, retinal disorders
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PEDF secretion
RPE-PEDF
PEDF concentration
OD reading by photometry
Growth factor secretion, retinal disorders
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VEGF secretion
RPE-VEGF
VEGF concentration
OD reading by photometry
Growth factor secretion, retinal disorders
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Phagocytosis assay
RPE-PHAG
Cell ratio intensity of staining
Fluorescence imaging
Phagocytosis, retinal disorders
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Melanin content
RPE-MEL
Concentration in melanin
OD475 reading photometry
Pigmentation
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Apoptosis
RPE-TUN
Cell ratio intensity of staining
TUNEL Fluorescent staining
Apoptosis
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Mitochondrial Oxidative stress
RPE-MSX
Cell ratio intensity of staining
Fluorescent staining
Oxidative stress response at mitochondria level
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Cellular oxidative stress
RPE-CRX
Cell ratio intensity of staining
Fluorescent staining
Oxidative stress response at cell level
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Cell proliferation/Survival
RPE-LCC
Nb live/dead cells
Calcin AM - Annexin V*
Cell proliferation - Survival
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