Retinal-related assays

PCi-RPE (retinal pigment epithelium)
cells display the full phenotypic, molecular and functionalcharacteristics of in situ RPE with the advantage of unrestricted availability.

RPE-based bioassays

Name

Ref.

Endpoint

Method

Biological question

mRNA expression

RPE-RNA

Relative expression level. All genes

RT-qPCR

Retinal disorders

TYRP1 expression

RPE-TYRP1

TYRP1 immunolabeling

Immuno-fluorescence

Pigmentation

PMEL17 expression

RPE-PMEL17

PMEL17 immunolabeling

Immuno-fluorescence

Pigmentation

ZO-1 expression

RPE-ZO1

ZO-1 immunolabeling

Immuno-fluorescence

Tight-junction, epithelial barrier

MITF expression

RPE-MITF

MITF immunolabeling

Immuno-fluorescence

Key transcription factor

Outer retinal barrier

RPE-ORB

Resistance in Ohm/cm2

Epithelial Volt/Ohm meter

Epithelial barrier, retinal disorders

RPE-PEDF

PEDF concentration

OD reading by photometry

Growth factor secretion, retinal disorders

RPE-VEGF

VEGF concentration

OD reading by photometry

Growth factor secretion, retinal disorders

Phagocytosis assay

RPE-PHAG

Cell ratio intensity of staining

Fluorescence imaging

Phagocytosis, retinal disorders

Melanin content

RPE-MEL

Concentration in melanin

OD475 reading photometry

Pigmentation

RPE-TUN

Cell ratio intensity of staining

TUNEL Fluorescent staining

Apoptosis

Mitochondrial Oxidative stress

RPE-MSX

Cell ratio intensity of staining

Fluorescent staining

Oxidative stress response at mitochondria level

Cellular oxidative stress

RPE-CRX

Cell ratio intensity of staining

Fluorescent staining

Oxidative stress response at cell level

Cell proliferation/Survival

RPE-LCC

Nb live/dead cells

Calcin AM - Annexin V*

Cell proliferation - Survival

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